研究業績 of Nakayama Lab

Mitsumori R, Shinmyozu K, Nakayama JI, Uchida H, Oki M.
Gic1 is a novel heterochromatin boundary protein in vivo.
Genes Genet Syst. 27301280 DOI: 10.1266/ggs.15-00070 PDF

Shimojo H, Kawaguchi A, Oda T, Hashiguchi N, Omori S, Moritsugu K, Kidera A, Hiragami-Hamada K, Nakayama JI, Sato M & Nishimura Y
Extended string-like binding of the phosphorylated HP1α N-terminal tail to the lysine 9-methylated histone H3 tail.
Sci Rep.6:22527. doi: 10.1038/srep22527. (2016) PDF

Nishibuchi G, Machida S, Osakabe A, Murakoshi H, Hiragami-Hamada K, Nakagawa R, Fischle W, Nishimura Y, Kurumizaka H, Tagami H, Nakayama JI.
N-terminal phosphorylation of HP1α increases its nucleosome-binding specificity.
Nucleic Acids Res. 42(20):12498-511. (2014) PDF

Nishibuchi G, Shibata Y, Hayakawa T, Hayakawa N, Ohtani Y, Shinmyozu K, Tagami H, Nakayama JI.
Physical and Functional Interactions between the Histone H3K4 Demethylase KDM5A and the Nucleosome Remodeling and Deacetylase (NuRD) Complex.
J Biol Chem. 289(42):28956-70 (2014) PDF

Hountondji C, Bulygin K, Créchet JB, Woisard A, Tuffery P, Nakayama J, Frolova L, Nierhaus KH, Karpova G, Baouz S.
The CCA-end of P-tRNA Contacts Both the Human RPL36AL and the A-site Bound Translation Termination Factor eRF1 at the Peptidyl Transferase Center of the Human 80S Ribosome.
Open Biochem J. 8:52-67. (2014) PDF

Fawcett JA, Iida T, Takuno S, Sugino RP, Kado T, Kugou K, Mura S, Kobayashi T, Ohta K, Nakayama J, Innan H.
Population Genomics of the Fission Yeast Schizosaccharomyces pombe.
PLoS One. 9(8):e104241 (2014) PDF

Nishibuchi G and Nakayama JI.
Biochemical and structural properties of Heterochromatin Protein 1: understanding its role in chromatin assembly
J. Biochem 156(1):11-20 (2014) PDF

Kato H, Okazaki K, Iida T, Nakayama J, Murakami Y, Urano T.
Spt6 prevents transcription-coupled loss of posttranslationally modified histone H3.
Sci Rep. 3:2186 (2013) PDF

Mano Y, Kobayashi T, Nakayama J, Uchida H, Oki M.
Correlation of Epigenetic Switching between Multiple Single Cell Visualization of Yeast Gene Expression Shows Heterochromatic Regions through Multiple Generations
PLOS Biology. (7):e1001601 (2013) PDF

Kamata K, Hatanaka A, Goswami G, Shinmyozu K, Nakayama JI, Urano T, Hatashita M, Uchida H, Oki M.
C-terminus of the Sgf73 subunit of SAGA and SLIK is important for retention in the larger complex and for heterochromatin boundary function.
Genes cells. (9):823-37 (2013)

Kawakami, K., Hayashi, A., Nakayama, J., and Murakami, Y.
A novel RNAi protein, Dsh1, assembles RNAi machinery on chromatin to amplify heterochromatic siRNA.
Genes Dev. 26, 1811-1824 (2012) PDF

Ishida, M., Shimojo, H., Hayashi, A., Kawaguchi, R., Ohtani, Y., Uegaki, K., Nishimura, Y. and Nakayama, J.
Intrinsic Nucleic Acid-Binding Activity of Chp1 Chromodomain Is Required for Heterochromatic Gene Silencing.
Mol. Cell. 47, 228-41 (2012) PDF, Preview

Shiomi, Y., Ishii, T., Hayashi, A., Shinmyozu, K., Nakayama, J., Sugasawa, K. and Nishitani, H.
Two different RFC proteins, Ctf18 and RFC1 separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV-irradiation.
Mol Cell Biol. 32, 2279-88 (2012) PDF

Hayashi, A., Ishida, M., Kawaguchi, R., Urano, T., Murakami, Y., and Nakayama, J.
Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.
Proc Natl Acad Sci U S A. 109, 6159-64 (2012) PDF

Hatanaka, A., Chen, B., Sun, J.Q., Mano, Y., Funakoshi, M., Kobayashi, H., Ju, Y., Mizutani, T., Shinmyozu, K., Nakayama, J., Miyamoto, K., Uchida, H. and Oki, M.
Fub1p, a novel protein isolated by boundary screening, binds the proteasome complex.
Genes & Genetic Systems. 86, 305-314 (2011) PDF

Goto, D.B. and Nakayama, J.
RNA and epigenetic silencing: Insight from fission yeast.
Dev Growth Differ. 54, 129-141 (2011) PDF

Kitano, E., Hayashi, A., Kanai, D., Shinmyozu, K. and Nakayama, J.
Roles of fission yeast Grc3 in ribosomal RNA processing and heterochromatic gene silencing.
J. Biol. Chem. 286, 15391-402 (2011)　PDF, Supplemental data

Hiragami-Hamada, K., Shinmyozu, K., Hamada, D., Tatsu, Y., Uegaki, S., Fujiwara, S. and Nakayama, J.
N-terminal phosphorylation of HP1α promotes its chromatin binding.
Mol. Cell. Biol. 31, 1186-200 (2011)　PDF

Hayakawa, T. and Nakayama, J.
Physiological roles of Class I HDAC complex and Histone demethylase.
J. Biomed. Biotechnol. (2011)　 PDF

Shirai, A., Sadaie, M., Shinmyozu, K., and Nakayama, J.
Methylation of ribosomal protein L42 regulates ribosomal function and stress-adapted cell growth.
J. Biol. Chem. 285, 22448-60. (2010) 　PDF, Supplemental data

Hayakawa, T., Zhang, F., Hayakawa, N., Ohtani, Y., Shinmyozu, K., Nakayama, J.*, and Andressen, PR.*
MRG15 directly binds to PALB2 and stimulates homology-directed repair of chromosomal breaks.
J. Cell Sci. 123, 1124-30. (*corresponding authors) (2010)　 PDF, Supplemental data

Shimada, Y., Fukuda, W., Akada, Y., Ishida, M., Nakayama, J., Imanaka, T., and Fujiwara S.
Property of cold inducible DEAD-box RNA helicase in hyperthermophilic archaea.
Biochem. Biophys. Res. Commun. 389, 622-7. (2009)　 PDF

Yamada, Y., Fukuda, W., Hirooka, K., Hiromoto, T., Nakayama, J., Imanaka, T., Fukusaki, E., and Fujiwara, S.
Efficient in vitro synthesis of cis-polyisoprenes using a thermostable cis-prenyltransferase from a hyperthermophilic archaeon Thermococcus kodakaraensis.
J. Biotechnol. 143, 151-6. (2009) 　PDF

Okada, S., Nagabuchi, M., Takamura, Y., Nakagawa, T., Shinmyozu, K., Nakayama, J., and Tanaka, K.
Reconstitution of Arabidopsis thaliana SUMO pathways in E. coli: functional evaluation of SUMO machinery proteins and mapping of SUMOylation sites by mass spectrometry.
Plant Cell Physiol. 50, 1049-61. (2009) 　PDF, Supplemental data

Shimada, A., Dohke, K., Sadaie, M., Shinmyozu, K., Nakayama, J., Urano, T., and Murakami, Y.
Phosphorylation of Swi6/HP1 regulates transcriptional gene silencing at heterochromatin.
Genes Dev. 23, 18-23 (2009) 　PDF, Supplemental data

Hayashi, M.T., Takahashi, T.S., Nakagawa, T., Nakayama, J., and Masukata, H.
The heterochromatin protein Swi6/HP1 activates replication origins at pericentromeres and silent mating-type locus.
Nat. Cell Biol. 11, 357-62 (2009) 　PDF, Supplemental data

Sadaie, M., Kawaguchi, R., Ohtani, Y., Arisaka, F., Tanaka, K., Shirahige, K., and Nakayama, JI.
Balance between distinct HP1 family proteins controls heterochromatin assembly in fission yeast.
Mol. Cell. Biol. 28, 6973-88 (2008) 　PDF, Supplemental data

Sawada, T., Nishiyama, C., Kishi, T., Sasazuki, T., Komazawa-Sakon, S., Xue, X., Piao, JH., Ogata, H., Nakayama, J., Taki, T., Hayashi, Y., Watanabe, M., Yagita, H., Okumura, K., and Nakano, H.
Fusion of One twenty-two to BSAC (Basic, SAP, and coiled-coil domain) results in aberrant upregulation of transcriptional activity.
J. Biol. Chem. 283, 26820-26828 (2008)　 PDF, Supplemental data

Iida, T., Nakayama, J.,and Moazed, D.
siRNA-Mediated Heterochromatin Establishment Requires HP1 and Is Associated with Antisense Transcription.
Mol. Cell. 31, 178-189 (2008) 　PDF, Supplemental data

Sadaie, M., Shinmyozu, K.,and Nakayama, J.
A conserved SET-domain methyltransferase, Set11, modifies ribosomal protein Rpl12 in fission yeast.
J. Biol. Chem. 283, 7185-7195 (2008) 　PDF, Supplemental data

Chikashige, Y., Tsutsumi, C., Okamasa, K., Yamane, M., Nakayama, J., Niwa, O., Haraguchi, T., and Hiraoka, Y.
Gene expression and distribution of Swi6 in partial aneuploids of the fission yeast Schizosaccharomyces pombe.
Cell Struct. Funct. 32, 149-161 (2008) 　PDF

Fujiwara, S., Yamanaka, A., Yamada, Y., Hirooka, K., Higashibata, H., Fukuda, W., Nakayama, JI., Imanaka, T., and Fukusaki, EI.
Efficient synthesis of trans-polyisoprene compounds using two thermostable enzymes in an organic-aqueous dual-liquid phase system.
Biochem. Biophys. Res. Commun. 365, 118-123 (2008) 　PDF

Hayakawa, T., Ohtani,Y., Hayakawa, N., Shinmyozu, K., Saito, M., Ishikawa, F., and Nakayama, J.
RBP2 is an MRG15 complex component and downregulates intragenic histone H3 lysine 4 methylation.
Genes Cells. 12, 811-826 (2007) 　PDF

Takasaki, N., Kurokawa, D., Nakayama, R., Nakayama, JI., and Aizawa,S.
Acetylated YY1 regulates Otx2 expression in anterior neuroectoderm at two cis-sites 90 kb apart.
EMBO J. 26, 1649-1659 (2007) 　PDF

Buker, S.M., Iida, T., Buhler, M., Villen, J., Gygi, S.P., Nakayama, J., and Moazed, D.
Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast.
Nat. Struct. Mol. Biol. 14, 200-207 (2007) 　PDF

Takasaki, T., Liu, Z., Habara, Y., Nishiwaki, K., Nakayama, J., Inoue, K., Sakamoto, H., and Strome, S.
MRG-1, an autosome-associated protein, silences X-linked genes and protects germline immortarity in C. elegans.
Development. 134,757-67 (2007)　 PDF

Iida, T., Kawaguchi, R., and Nakayama, J.
Conserved ribonuclease, Eri1, negatively regulates heterochromatin assembly in fission yeast.
Curr. Biol. 16, 1459-1464 (2006)　 PDF, Supplemental data

Tsumura, A., Hayakawa, T., Kumaki, Y., Takebayashi, S., Sakaue, M., Matsuoka, C., Shimotohno, K., Ishikawa, F., Li, E., Ueda, H. R., Nakayama, J., and Okano, M.
Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b.
Genes Cells. 11, 805-814. (2006) 　PDF

Nishijima, H., Nakayama,J., Yoshioka, T., Kusano, A., Nishitani, H., Shibahara, K., and Nishimoto, T.
Nuclear RanGAP is required for the heterochromatin assembly and reciprocally regulated by histones and Clr4 histone methyltransferase in S. pombe.
Mol. Biol. Cell. 17, 2524-2536. (2006)　 PDF

Sadaie, M., Iida, T., Urano, T., and Nakayama, J.
A chromodomain protein, Chp1, is required for the establishment of heterochromatin in fission yeast.
EMBO J. 23, 3825-3835. (2004) 　PDF

Nakayama, J., Xiao, G., Noma, K., Malikzay, A., Bjerling, P., Ekwall, K., Kobayashi, R., and Grewal, S.I.S.
Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genome integrity.
EMBO J. 22, 2776-2787. (2003)　 PDF

Tamaru, H., Zhang, X., McMillen, D., Singh, P., Nakayama, J., Grewal, S., Allis, D., Cheng, X., and Selker, E.U.
Trimethylation of histone H3 lysine-9 associated with methylated DNA in Neurospora crassa.
Nature Genet. 34, 75-79. (2003)　 PDF

Nakagawa, H., Lee, J.-K., Hurwitz, J., Allshire, R.C., Nakayama, J., Grewal, S.I.S., Tanaka, K., and Murakami, Y.
Fission yeast CENP-B homologs nucleate centromeric heterochromatin by prompting heterochromatin-specific histone tail modifications.
Genes Dev. 16, 1766-1778. (2002)　 PDF

Nakayama, J., and Ishikawa, F.
Stretch PCR assay.
Methods Mol. Biol. 191, 125-136. (2002) 　 PDF

Nakayama, J., Rice, J.C., Strahl, B.D., Allis, C.D., and Grewal, S.I.S.
Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly.
Science. 292, 110-113. (2001) 　PDF, Supplemental data

Nakayama, J., Allshire, R.C., Klar, A.J.S., and Grewal, S.I.S
A role for DNA polymerase alpha in epigenetic control of transcriptional silencing in fission yeast.
EMBO J. 20, 2857-2866. (2001)　 PDF

Nakayama, J., Klar, A.J.S., and Grewal, S.I.S.
A chromodomain protein, Swi6, performs imprinting functions in fission yeast during mitosis and miosis.
Cell. 101, 307-317. (2000)　 PDF

Kawakami, Y., Kitamoto, M., Nakanishi, T., Yasui, W., Tahara, E., Nakayama, J., Ishikawa, F., Tahara, H., Ide, T., and Kajiyama, G.
Immuno-histochemical detection of human telomerase reverse transcriptase in human liver tissues.
Oncogene. 19, 3888-3893. (2000)　 PDF

Poremba, C., Scheel, C., Hero, B., Christiansen, H., Schaefer, K.-L., Nakayama, J., Berthold, F., Juergens, H., Boecker, W., and Dockhorn-Dworniczak, B.
Telomerase activity and telomerase subunits gene expression patterns in neuroblastoma: A molecular and immunohistochemical study establishing prognostic tools for fresh-frozen and paraffin-embedded tissues.
J. Clin. Oncol. 18, 2582-2592. (2000)　 PDF

Yuan, X., Ishibashi, S., Hatakeyama, S., Saito, M., Nakayama, J., Nikaido, R., Haruyama, T., Watanabe, Y., Iwata, H., Iida, M., Sugimura, H., Yamada, N., and Ishikawa, F.
Presence of telomeric G-strand tails in the telomerase catalytic subunit TERT knockout mice.
Genes Cells. 4, 563-572. (1999)　 PDF

Yasui, W., Tahara, E., Tahara, H., Fujimoto, J., Naka, K., Nakayama, J., Ishikawa, F., Ide, T., and Tahara, E
Immunohistochemical detection of human telomerase reverse transcriptase in normal mucosa and precancerous lesions of the stomach.
Jpn. J. Cancer Res. 90, 589-595. (1999)

Tahara, H., Yasui, W., Tahara, E., Fujimoto, J., Ito, K., Tamai, K., Nakayama, J., Ishikawa, F., Tahara, E., and Ide, T.
Immuno-histochemical detection of human telomerase catalytic component, hTERT, in human colorectal tumor and non-tumor tissue sections.
Oncogene. 18, 1561-1567. (1999)　 PDF

Yasui, W., Tahara, H., Tahara, E., Fujimoto, J., Nakayama, J., Ishikawa, F., Ide, T., and Tahara, E.
Expression of telomerase catalytic component, telomerase reverse transcriptase, in human gastric carcinomas.
Jpn. J. Cancer Res. 89, 1099-1103. (1998)

Nakayama, J., Tahara, H., Tahara, E., Saito, M., Ito, K., Nakamura, H., Nakanishi, T., Tahara, E., Ide, T., and Ishikawa, F.
Telomerase activation by hTRT in human normal fibroblast and hepatocellular carcinomas.
Nature Genet. 18, 65-68. (1998)　 PDF

Saito, T., Matsuda, Y., Suzuki, T., Hayashi, A., Yuan, X., Saito, M., Nakayama, J., Hori, T., and Ishikawa, F.
Comparative gene mapping of the human and mouse TEP1 genes, which encode one protein component of telomerases.
Genomics. 46, 46-50. (1997) 　PDF

Nakayama, J., Saito, M., Nakamura, H., Matsuura, A., and Ishikawa, F.
TLP1: A gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family.
Cell. 88, 875-884. (1997)　 PDF

Tatematsu, K., Nakayama, J., Danbara, M., Sato, H., Omine, M., and Ishikawa, F.
A novel quantitative 'stretch PCR assay', that detects a drastic increase in telomerase activity during the progression of myeloid leukemias.
Oncogene. 13, 2265-2274. (1996)

Kajita, Y., Nakayama, J., Aizawa, M., and Ishikawa, F.
The UUAG-specific RNA Binding Protein, hnRNP D0: Common Modular Structure and Binding Properties of 2xRBD-Gly Family.
J. Biol. Chem. 270, 22167-22175. (1995)　 PDF

西淵剛平・中山潤一「ヒト培養細胞を用いたタンパク質複合体精製のためのアフィニティータグの検討」蛋白質科学会アーカイブ　(2010） PDF

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中山潤一「ヘテロクロマチン構造の形成とサイレンシング」生化学　vol.85(7), p565-70 日本生化学会　(2013) PDF

中山潤一「ヘテロクロマチン構造からみる非コードDNAと染色体」実験医学　2012年9月号　30(14): 2221-2227　PDF

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中山潤一「高次クロマチン構造とノンコーディングRNA」細胞工学　2011年7月号　30(7): 699-705 （2011)

中山潤一「3章　概観と概念」　『エピジェネティクス(堀越正美 監訳）』　27-73　培風館　(2010)　PDF

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中山潤一「高次クロマチンの形成機構とエピジェネティック制御」蛋白質核酸酵素　2008年6月号　53(7):　801-808 (2008)　PDF

石田真由美・中山潤一「小分子RNAとクロマチン動態」無敵のバイオテクニカルシリーズ『RNA実験ノート下（稲田利文・塩見春彦）』16-17 (2008)　PDF

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中山潤一「ヘテロクロマチン-構造形成と維持の分子メカニズム-」医学のあゆみ　208: 811-816 (2004)　PDF

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中山潤一「ヒストン修飾酵素」 クロマチンと遺伝子発現制御、堀越正美編、p81-90、シュプリンガー・フェアラーク・東京 (2003)　PDF

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中山潤一、石川冬木 「テロメラーゼ」 RNA研究の最前線、志村令朗・渡辺公綱編、シュプリンガー・フェアラーク・東京 (2000)

中山潤一、石川冬木 「テロメラーゼの活性化と細胞老化」実験医学 7月号 16: 1284-1287 (1998)

中山潤一、石川冬木 「テロメラーゼの分子構造と活性制御機構」 日本臨牀 5月号 56: 1102- 1107 (1998)

中山潤一、石川冬木 「哺乳類テロメラーゼ -構成蛋白質TLP1を中心に-」 実験医学 10月号 15: 1823-1828 (1997)

石川冬木、中山潤一 「哺乳類テロメラーゼ蛋白質 TLP1」 蛋白質・核酸・酵素 7月号 42: 57-69 (1997)

中山潤一、石川冬木 「Stretch PCRを利用したテロメラーゼ活性の測定法」 実験医学 増刊「新PCRとその応用」15: 60-65 (1997)

中山潤一、石川冬木 「テロメラーゼ」 最新医学 7月号　51: 124-130 (1996)