2016
Shimojo H, Kawaguchi A, Oda T, Hashiguchi N, Omori S, Moritsugu K, Kidera A, Hiragami-Hamada K, Nakayama JI, Sato M & Nishimura Y
Extended string-like binding of the phosphorylated HP1α N-terminal tail to the lysine 9-methylated histone H3 tail.
Sci Rep.6:22527. doi: 10.1038/srep22527. (2016) PDF
2014
Nishibuchi G, Machida S, Osakabe A, Murakoshi H, Hiragami-Hamada K, Nakagawa R, Fischle W, Nishimura Y, Kurumizaka H, Tagami H, Nakayama JI.
N-terminal phosphorylation of HP1α increases its nucleosome-binding specificity.
Nucleic Acids Res. in press (2014) PDF
Nishibuchi G, Shibata Y, Hayakawa T, Hayakawa N, Ohtani Y, Shinmyozu K, Tagami H, Nakayama JI.
Physical and Functional Interactions between the Histone H3K4 Demethylase KDM5A and the Nucleosome Remodeling and Deacetylase (NuRD) Complex.
J Biol Chem. 289(42):28956-70 (2014) PDF
Hountondji C, Bulygin K, Créchet JB, Woisard A, Tuffery P, Nakayama J, Frolova L, Nierhaus KH, Karpova G, Baouz S.
The CCA-end of P-tRNA Contacts Both the Human RPL36AL and the A-site Bound Translation Termination Factor eRF1 at the Peptidyl Transferase Center of the Human 80S Ribosome.
Open Biochem J. 8:52-67. (2014) PDF
Fawcett JA, Iida T, Takuno S, Sugino RP, Kado T, Kugou K, Mura S, Kobayashi T, Ohta K, Nakayama J, Innan H.
Population Genomics of the Fission Yeast Schizosaccharomyces pombe.
PLoS One. 9(8):e104241 (2014) PDF
Nishibuchi G and Nakayama JI.
Biochemical and structural properties of Heterochromatin Protein 1: understanding its role in chromatin assembly
J. Biochemin press (2014) PDF
2013
Kato H, Okazaki K, Iida T, Nakayama J, Murakami Y, Urano T.
Spt6 prevents transcription-coupled loss of posttranslationally modified histone H3.
Sci Rep. 3:2186 (2013) PDF
Yasunobu Mano, Tetsuya J. Kobayashi, Jun-ichi Nakayama, Hiroyuki Uchida, Masaya Oki
Correlation of Epigenetic Switching between Multiple Single Cell Visualization of Yeast Gene Expression Shows Heterochromatic Regions through Multiple Generations
PLOS Biology. (7):e1001601 (2013) PDF
Kamata K, Hatanaka A, Goswami G, Shinmyozu K, Nakayama JI, Urano T, Hatashita M, Uchida H, Oki M.
C-terminus of the Sgf73 subunit of SAGA and SLIK is important for retention in the larger complex and for heterochromatin boundary function.
Genes cells. (9):823-37 (2013)
2012
Kawakami, K., Hayashi, A., Nakayama, J., and Murakami, Y.
A novel RNAi protein, Dsh1, assembles RNAi machinery on chromatin to amplify heterochromatic siRNA.
Genes Dev, 26, 1811-1824 (2012) PDF
Ishida, M., Shimojo, H., Hayashi, A., Kawaguchi, R., Ohtani, Y., Uegaki, K., Nishimura, Y. and Nakayama, J.
Intrinsic Nucleic Acid-Binding Activity of Chp1 Chromodomain Is Required for Heterochromatic Gene Silencing.
Mol. Cell. 47, 228-41 (2012) PDF. Preview
Shiomi, Y., Ishii, T., Hayashi, A., Shinmyozu, K., Nakayama, J., Sugasawa, K. and Nishitani, H.
Two different RFC proteins, Ctf18 and RFC1 separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV-irradiation.
Mol Cell Biol. 32, 2279-88 (2012) PDF
Hayashi, A., Ishida, M., Kawaguchi, R., Urano, T., Murakami, Y., and Nakayama, J.
Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.
Proc Natl Acad Sci U S A. 109, 6159-64 (2012) PDF
2011
Hatanaka, A., Chen, B., Sun, J.Q., Mano, Y., Funakoshi, M., Kobayashi, H., Ju, Y., Mizutani, T., Shinmyozu, K., Nakayama, J., Miyamoto, K., Uchida, H. and Oki, M.
Fub1p, a novel protein isolated by boundary screening, binds the proteasome complex.
Genes & Genetic Systems. 86, 305-314 (2011) PDF
Goto, D.B. and Nakayama, J.
RNA and epigenetic silencing: Insight from fission yeast.
Dev Growth Differ. 54, 129-141 (2011) PDF
Kitano, E., Hayashi, A., Kanai, D., Shinmyozu, K. and Nakayama, J.
Roles of fission yeast Grc3 in ribosomal RNA processing and heterochromatic gene silencing.
J. Biol. Chem. 286, 15391-402 (2011) PDF, Supplemental data
Hiragami-Hamada, K., Shinmyozu, K., Hamada, D., Tatsu, Y., Uegaki, S., Fujiwara, S. and Nakayama, J.
N-terminal phosphorylation of HP1α promotes its chromatin binding.
Mol. Cell. Biol. 31, 1186-200 (2011) PDF
Hayakawa, T. and Nakayama, J.
Physiological roles of Class I HDAC complex and Histone demethylase.
J. Biomed. Biotechnol. (2011) PDF
2010
Shirai, A., Sadaie, M., Shinmyozu, K., and Nakayama, J.
Methylation of ribosomal protein L42 regulates ribosomal function and stress-adapted cell growth.
J. Biol. Chem. 285, 22448-60. (2010) PDF, Supplemental data
Hayakawa, T., Zhang, F., Hayakawa, N., Ohtani, Y., Shinmyozu, K., Nakayama, J.*, and Andressen, PR.*
MRG15 directly binds to PALB2 and stimulates homology-directed repair of chromosomal breaks.
J. Cell Sci. 123, 1124-30. (*corresponding authors) (2010) PDF, Supplemental data
2009
Shimada, Y., Fukuda, W., Akada, Y., Ishida, M., Nakayama, J., Imanaka, T., and Fujiwara S.
Property of cold inducible DEAD-box RNA helicase in hyperthermophilic archaea.
Biochem. Biophys. Res. Commun. 389, 622-7. (2009) PDF
Yamada, Y., Fukuda, W., Hirooka, K., Hiromoto, T., Nakayama, J., Imanaka, T., Fukusaki, E., and Fujiwara, S.
Efficient in vitro synthesis of cis-polyisoprenes using a thermostable cis-prenyltransferase from a hyperthermophilic archaeon Thermococcus kodakaraensis.
J. Biotechnol. 143, 151-6. (2009) PDF
Okada, S., Nagabuchi, M., Takamura, Y., Nakagawa, T., Shinmyozu, K., Nakayama, J., and Tanaka, K.
Reconstitution of Arabidopsis thaliana SUMO pathways in E. coli: functional evaluation of SUMO machinery proteins and mapping of SUMOylation sites by mass spectrometry.
Plant Cell Physiol. 50, 1049-61. (2009) PDF, Supplemental data
Shimada, A., Dohke, K., Sadaie, M., Shinmyozu, K., Nakayama, J., Urano, T., and Murakami, Y.
Phosphorylation of Swi6/HP1 regulates transcriptional gene silencing at heterochromatin.
Genes Dev. 23, 18-23 (2009) PDF, Supplemental data
Hayashi, M.T., Takahashi, T.S., Nakagawa, T., Nakayama, J., and Masukata, H.
The heterochromatin protein Swi6/HP1 activates replication origins at pericentromeres and silent mating-type locus.
Nat. Cell Biol. 11, 357-62 (2009) PDF, Supplemental data
2008
Sadaie, M., Kawaguchi, R., Ohtani, Y., Arisaka, F., Tanaka, K., Shirahige, K., and Nakayama, JI.
Balance between distinct HP1 family proteins controls heterochromatin assembly in fission yeast.
Mol. Cell. Biol. 28, 6973-88 (2008) PDF, Supplemental data
Sawada, T., Nishiyama, C., Kishi, T., Sasazuki, T., Komazawa-Sakon, S., Xue, X., Piao, JH., Ogata, H., Nakayama, J., Taki, T., Hayashi, Y., Watanabe, M., Yagita, H., Okumura, K., and Nakano, H.
Fusion of One twenty-two to BSAC (Basic, SAP, and coiled-coil domain) results in aberrant upregulation of transcriptional activity.
J. Biol. Chem. 283, 26820-26828 (2008) PDF, Supplemental data
Iida, T., Nakayama, J.,and Moazed, D.
siRNA-Mediated Heterochromatin Establishment Requires HP1 and Is Associated with Antisense Transcription.
Mol. Cell. 31, 178-189 (2008) PDF, Supplemental data
Sadaie, M., Shinmyozu, K.,and Nakayama, J.
A conserved SET-domain methyltransferase, Set11, modifies ribosomal protein Rpl12 in fission yeast.
J. Biol. Chem. 283, 7185-7195 (2008) PDF, Supplemental data
Chikashige, Y., Tsutsumi, C., Okamasa, K., Yamane, M., Nakayama, J., Niwa, O., Haraguchi, T., and Hiraoka, Y.
Gene expression and distribution of Swi6 in partial aneuploids of the fission yeast Schizosaccharomyces pombe.
Cell Struct. Funct. 32, 149-161 (2008) PDF
Fujiwara, S., Yamanaka, A., Yamada, Y., Hirooka, K., Higashibata, H., Fukuda, W., Nakayama, JI., Imanaka, T., and Fukusaki, EI.
Efficient synthesis of trans-polyisoprene compounds using two thermostable enzymes in an organic-aqueous dual-liquid phase system.
Biochem. Biophys. Res. Commun. 365, 118-123 (2008) PDF
2007
Hayakawa, T., Ohtani,Y., Hayakawa, N., Shinmyozu, K., Saito, M., Ishikawa, F., and Nakayama, J.
RBP2 is an MRG15 complex component and downregulates intragenic histone H3 lysine 4 methylation.
Genes Cells. 12, 811-826 (2007) PDF
Takasaki, N., Kurokawa, D., Nakayama, R., Nakayama, JI., and Aizawa,S.
Acetylated YY1 regulates Otx2 expression in anterior neuroectoderm at two cis-sites 90 kb apart.
EMBO J. 26, 1649-1659 (2007) PDF
Buker, S.M., Iida, T., Buhler, M., Villen, J., Gygi, S.P., Nakayama, J., and Moazed, D.
Two different Argonaute complexes are required for siRNA generation and heterochromatin assembly in fission yeast.
Nat. Struct. Mol. Biol. 14, 200-207 (2007) PDF
Takasaki, T., Liu, Z., Habara, Y., Nishiwaki, K., Nakayama, J., Inoue, K., Sakamoto, H., and Strome, S.
MRG-1, an autosome-associated protein, silences X-linked genes and protects germline immortarity in C. elegans.
Development. 134,757-67 (2007) PDF
2006
Iida, T., Kawaguchi, R., and Nakayama, J.
Conserved ribonuclease, Eri1, negatively regulates heterochromatin assembly in fission yeast.
Curr. Biol. 16, 1459-1464 (2006) PDF, Supplemental data
Tsumura, A., Hayakawa, T., Kumaki, Y., Takebayashi, S., Sakaue, M., Matsuoka, C., Shimotohno, K., Ishikawa, F., Li, E., Ueda, H. R., Nakayama, J., and Okano, M.
Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b.
Genes Cells. 11, 805-814. (2006) PDF
Nishijima, H., Nakayama,J., Yoshioka, T., Kusano, A., Nishitani, H., Shibahara, K., and Nishimoto, T.
Nuclear RanGAP is required for the heterochromatin assembly and reciprocally regulated by histones and Clr4 histone methyltransferase in S. pombe.
Mol. Biol. Cell. 17, 2524-2536. (2006) PDF
2004
Sadaie, M., Iida, T., Urano, T., and Nakayama, J.
A chromodomain protein, Chp1, is required for the establishment of heterochromatin in fission yeast.
EMBO J. 23, 3825-3835. (2004) PDF
2003
Nakayama, J., Xiao, G., Noma, K., Malikzay, A., Bjerling, P., Ekwall, K., Kobayashi, R., and Grewal, S.I.S.
Alp13, an MRG family protein, is a component of fission yeast Clr6 histone deacetylase required for genome integrity.
EMBO J. 22, 2776-2787. (2003) PDF
Tamaru, H., Zhang, X., McMillen, D., Singh, P., Nakayama, J., Grewal, S., Allis, D., Cheng, X., and Selker, E.U.
Trimethylation of histone H3 lysine-9 associated with methylated DNA in Neurospora crassa.
Nature Genet. 34, 75-79. (2003) PDF
2002
Nakagawa, H., Lee, J.-K., Hurwitz, J., Allshire, R.C., Nakayama, J., Grewal, S.I.S., Tanaka, K., and Murakami, Y.
Fission yeast CENP-B homologs nucleate centromeric heterochromatin by prompting heterochromatin-specific histone tail modifications.
Genes Dev. 16, 1766-1778. (2002) PDF
Nakayama, J., and Ishikawa, F.
Stretch PCR assay.
Methods Mol. Biol. 191, 125-136. (2002) PDF
2001
Nakayama, J., Rice, J.C., Strahl, B.D., Allis, C.D., and Grewal, S.I.S.
Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly.
Science. 292, 110-113. (2001) PDF, Supplemental data
Nakayama, J., Allshire, R.C., Klar, A.J.S., and Grewal, S.I.S
A role for DNA polymerase alpha in epigenetic control of transcriptional silencing in fission yeast.
EMBO J. 20, 2857-2866. (2001) PDF
2000
Nakayama, J., Klar, A.J.S., and Grewal, S.I.S.
A chromodomain protein, Swi6, performs imprinting functions in fission yeast during mitosis and miosis.
Cell. 101, 307-317. (2000) PDF
Kawakami, Y., Kitamoto, M., Nakanishi, T., Yasui, W., Tahara, E., Nakayama, J., Ishikawa, F., Tahara, H., Ide, T., and Kajiyama, G.
Immuno-histochemical detection of human telomerase reverse transcriptase in human liver tissues.
Oncogene. 19, 3888-3893. (2000) PDF
Poremba, C., Scheel, C., Hero, B., Christiansen, H., Schaefer, K.-L., Nakayama, J., Berthold, F., Juergens, H., Boecker, W., and Dockhorn-Dworniczak, B.
Telomerase activity and telomerase subunits gene expression patterns in neuroblastoma: A molecular and immunohistochemical study establishing prognostic tools for fresh-frozen and paraffin-embedded tissues.
J. Clin. Oncol. 18, 2582-2592. (2000) PDF
1999
Yuan, X., Ishibashi, S., Hatakeyama, S., Saito, M., Nakayama, J., Nikaido, R., Haruyama, T., Watanabe, Y., Iwata, H., Iida, M., Sugimura, H., Yamada, N., and Ishikawa, F.
Presence of telomeric G-strand tails in the telomerase catalytic subunit TERT knockout mice.
Genes Cells. 4, 563-572. (1999) PDF
Yasui, W., Tahara, E., Tahara, H., Fujimoto, J., Naka, K., Nakayama, J., Ishikawa, F., Ide, T., and Tahara, E
Immunohistochemical detection of human telomerase reverse transcriptase in normal mucosa and precancerous lesions of the stomach.
Jpn. J. Cancer Res. 90, 589-595. (1999)
Tahara, H., Yasui, W., Tahara, E., Fujimoto, J., Ito, K., Tamai, K., Nakayama, J., Ishikawa, F., Tahara, E., and Ide, T.
Immuno-histochemical detection of human telomerase catalytic component, hTERT, in human colorectal tumor and non-tumor tissue sections.
Oncogene. 18, 1561-1567. (1999) PDF
1998
Yasui, W., Tahara, H., Tahara, E., Fujimoto, J., Nakayama, J., Ishikawa, F., Ide, T., and Tahara, E.
Expression of telomerase catalytic component, telomerase reverse transcriptase, in human gastric carcinomas.
Jpn. J. Cancer Res. 89, 1099-1103. (1998)
Nakayama, J., Tahara, H., Tahara, E., Saito, M., Ito, K., Nakamura, H., Nakanishi, T., Tahara, E., Ide, T., and Ishikawa, F.
Telomerase activation by hTRT in human normal fibroblast and hepatocellular carcinomas.
Nature Genet. 18, 65-68. (1998) PDF
1997
Saito, T., Matsuda, Y., Suzuki, T., Hayashi, A., Yuan, X., Saito, M., Nakayama, J., Hori, T., and Ishikawa, F.
Comparative gene mapping of the human and mouse TEP1 genes, which encode one protein component of telomerases.
Genomics. 46, 46-50. (1997) PDF
Nakayama, J., Saito, M., Nakamura, H., Matsuura, A., and Ishikawa, F.
TLP1: A gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family.
Cell. 88, 875-884. (1997) PDF
1996
Tatematsu, K., Nakayama, J., Danbara, M., Sato, H., Omine, M., and Ishikawa, F.
A novel quantitative 'stretch PCR assay', that detects a drastic increase in telomerase activity during the progression of myeloid leukemias.
Oncogene. 13, 2265-2274. (1996)
1995
Kajita, Y., Nakayama, J., Aizawa, M., and Ishikawa, F.
The UUAG-specific RNA Binding Protein, hnRNP D0: Common Modular Structure and Binding Properties of 2xRBD-Gly Family.
J. Biol. Chem. 270, 22167-22175. (1995) PDF